![]() Thus ABTS has some advantageous over TMB and OPD in cases of large background results.Ģ,2′-Azino-bis(3-ethylben zthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications.ABTS is also used to monitor glucose concentrations in different solutions including blood serum.ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods. ![]() 2,2′-Azino-bis(3-ethylb enzothiazoline-6-sulfonic acid) diammonium salt has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).
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